Hygromycin.com, Antibiotics for Gene Selection and Cell Culture

Hygromycin B, is an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus. It is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. The resistance gene codes for a kinase (Hygromycin phosphotransferase, HPT) that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells. The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for the selection of resistant cells is 25-1000 ug/ml. Commonly used concentrations for selection are 200 ug/ml for mammalian cells, 20-200 ug/ml for plant cells & bacteria cells and 200-1000 ug/ml for fungi. Your optimum concentration should be tested experimentally.

Animal Use

This antibiotic was originally developed in the 1950's for veterinary use and is still added into swine and chicken feed as an anthelmintic or anti-worming agent.

Advantages of our antibiotics

As a leading OEM supplier and the larges producer of Hygromycin B worldwide, we provide:

Research Use

Animal Use

Advantages of our antibiotics