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The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for the selection of resistant cells is 25 - 1000µg/ml. commonly used concentrations for selection are 200 µg/ml mammalian cells, 20-200 µg/ml for plant cells & bacteria cells and 200 - 1000 µg/ml for fungi. Your optimum concentration should be tested experimentally.
When researchers want to focus on a primary gene of interest the use of antibiotic-resistance genes is extremely effective. Antibiotic-resistance genes are used as markers to identify the transfer of other genetic material (DNA) from one organism to another.
An antibiotic-resistance gene, when used as a marker, produces a protein that protects plants and organisms from a specific antibiotic. When exposed to the antibiotic, cells that have not taken up the new gene are unable to grow and thus die, while transformed cells continue to grow.
When transformed cells (cells with an antibiotic-resistance gene) and untransformed cells are exposed to an antibiotic, such as; G-418, Hygromycin B, Puromycin and Mycophenolic acid, only the transformed cells survive and grow. Researchers can therefore be confident that the surviving cells contain the gene of their primary research interest.